SPME: Theory and Practice
Presented by Janusz Pawliszyn, University of Waterloo
Tuesday, April 19, 2022 ◉ 1:00 pm to 3:00 pm EST
COURSE DESCRIPTION: In this course several microextraction technologies will be discussed, namely solid phase microextraction (SPME), thin film microextraction (TFME) and needle trap devices (NT). The main principles of these techniques, different calibration methods, coupling strategies to GC, LC, and MS, method development steps, and advantages and disadvantages of each microextraction approach will be covered. In addition, during this short course, recent advances, developments and applications of each technique in fields such as forensic, environmental, food and flavor, and clinical analysis will be described. Other topics that will be covered involve the application of SPME for ligand-receptor binding and plasma protein binding studies, in vivo sampling of freely moving animals for pharmacokinetic and metabolomic studies, direct tissue analysis, and automation of SPME and related technologies in high-throughput format.
LEARNING OBJECTIVES: Solventless technologies, Theoretical principles of SPME, TFME and NT, Interfaces, Calibration, Method development, Automation, In-vivo sampling, Drug and clinical analysis, Food, flavor and fragrance analysis, Environmental analysis, and Direct coupling of different SPME formats with mass spectrometry.
TARGET AUDIENCE: This course is designed for new and current SPME and TFME users. The topics that will be covered in this course will be of interest to analytical chemists, laboratory supervisors, scientists, and industry regulators in the environmental, food, pharmaceutical, clinical, cosmetic, and other fields.
CATEGORIES: Analytical Metrology, Environmental Analysis, Food, Gas Chromatography-Mass Spectrometry, Liquid Chromatography-Mass Spectrometry, Mass Spectrometry
About the Instructor
Professor Janusz Pawliszyn is the inventor of SPME and a professor in the Department of Chemistry, University of Waterloo, Canada. He is the author of over 700 journal publications, 20 book chapters, and over 600 invited/plenary lectures. He was also awarded the Pittcon Analytical Chemistry Award in 2017.