LCMS Method Development Strategies

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Presented by Priayanka Chitranshi
Shimadzu Scientific Instruments
Tuesday, October 5, 2021 12:00 PM EST
Cost: Free

Gearing up for LC-MS/MS method development

ABSTRACT: Liquid chromatography tandem mass spectrometry (LC-MS/MS) finds applications in wide range of industries including but not limited to pharmaceuticals/ biopharmaceuticals, food (including dietary supplements, cannabis, and hemp), forensics, clinical (including toxicology), chemical, and environmental. LC-MS/MS is considered the ‘gold standard’ for qualitative and quantitative analysis owing to the specificity, sensitivity, and wide linear dynamic range compared to other techniques. A typical analytical method can include one to several hundred analytes to be detected at low levels in complex matrices. Hence, method development is crucial for generation of a robust and reliable validated analytical method that can withstand inter- and intra- day variabilities between instruments and users. In this presentation, we will discuss crucial aspects of LC-MS/MS method development such as importance of appropriate mobile phase selection, Q1 and Q3 scans, tuning and optimization of the LCMS instrument, and MRM method development. Identifying and overcoming matrix-related signal suppression or enhancement will also be discussed.

1. Mobile phase selection
2. Tuning and optimization of mass spectrometry parameters
3. MRM method development
4. MRM ratio
5. Matrix-related signal suppression

Technicians, analysts, scientists who are looking for tips and tricks for generation of new or improvement of existing LC-MS/MS methods.

About Priayanka Chitranshi

Priyanka Chitranshi, Ph.D., is a LCMS Applications Scientist at Shimadzu Scientific Instruments, Columbia, MD.