Introduction to Chromatography of Proteins, Peptides, and Related Molecules
This is a beginner level course. It presumes some training and experience in analytical chemistry. Prior exposure to other separation techniques is useful. It is intended for scientists starting to analyze biological macromolecules and biopharmaceuticals. It surveys the range of available separation techniques to provide a basis for selecting one.
This full day program is an introduction to the separation techniques used to characterize biological macromolecules, with an emphasis on practical applications. The most useful modes for protein separations will be emphasized, and substantial description of peptide mapping will be presented. Topics include theory and principles of the separations. Rationale for choosing a technique will be systematically considered. Strategies for methods development will be presented. Analytical techniques that are often used in the same laboratories, including amino acid, glycan, and oligonucleotide analysis, will be introduced. The role of mass spectrometry in the analytical biochemistry laboratory will be described.