Analytical Spectroscopy Methods: UV-Vis Absorption & Fluorescence
Remember all that spectroscopy – electronic energy levels, vibrations and notation? UV-Vis absorption and fluorescence comprise the most widely used analytical tool set across disciplines. With applications ranging from fuel markers, food quality, pharmaceutical analysis, document and painting authentication and trace detection for explosives and even mineralogical screening. Providing motivation and aided by technology advances, instrumentation has become miniaturized, highly portable and comparatively inexpensive and most importantly easy to use. Yet despite spectral acquisition ease, proper application and interpretation still requires a sound knowledge of the underlying physical principles of the spectroscopy. Therein this course will review the fundamentals of each spectroscopic method and outline essential instrumentation components. Molecular analysis will serve as entry to real-world applications involving materials and complex mixtures. Interleaved with the lectures on each topic will be live demonstrations using Ocean Optics’ spectrometers for fluorescence, and absorbance. Live demonstrations bring relevance to the spectroscopic physical principles while illustrating state-of-the-art instrumentation such as LEDs and laser sources, fixed gratings, fiber optics and active visualization of analytical applications to manufacturing, quality control and environmental monitoring.
This course is designed for individuals who use or anticipate using absorption or fluorescence analyses in academic, governmental, or industrial laboratories: engineers, technicians, physical and biological scientists, clinicians, geologists, forensic scientists and technical managers.
1. Molecular energy levels, orbitals and types of electronic transitions
2. Beer’s law and uses
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6. Principles of Fluorescence
7. Quantum yield and lifetime
8. Fluorescence Intensity
Dr. Vander Wal is presently Professor of Energy Engineering and Materials Science and Engineering at Penn State University where he teaches courses in analytical methods, energy technology, nanotechnology, fuel science and environmental statistics. His research interests include the synthesis, characterization and application of nanomaterials for energy conversion, harvesting, storage and control (sensors) applications. The course is a distillation of these modules from his graduate level course on Analytical Methods that he teaches at Penn State.